Electrochemical genosensor for specific detection of the food-borne pathogen, Vibrio cholerae
Identifieur interne : 002560 ( Main/Exploration ); précédent : 002559; suivant : 002561Electrochemical genosensor for specific detection of the food-borne pathogen, Vibrio cholerae
Auteurs : Kim-Fatt Low [Malaisie] ; Kritsanaporn Chuenrangsikul [Thaïlande] ; Patsamon Rijiravanich [Thaïlande] ; Werasak Surareungchai [Thaïlande] ; Yean-Yean Chan [Malaisie]Source :
- World Journal of Microbiology and Biotechnology [ 0959-3993 ] ; 2012-04-01.
English descriptors
- KwdEn :
Abstract
Abstract: A disposable horseradish peroxidase (HRP)-based electrochemical genosensor was developed for chronoamperometric detection of single-stranded asymmetric lolB gene PCR amplicon (118 bp in length) of the food-borne pathogen, Vibrio cholerae. A two-step sandwich-type hybridization strategy using two specific probes was employed for specific detection of the target single-stranded DNA (ssDNA). The analytical performances of the detection platform have been evaluated using a synthetic ssDNA (ST3) which was identical to the target single-stranded amplicon and a total of 19 bacterial strains. Under optimal condition, ST3 was calibrated with a dynamic range of 0.4883–15.6250 nM. By coupling asymmetric PCR amplification, the probe-based electrochemical genosensor was highly specific to the target organism (100% specificity) and able to detect as little as 0.85 ng/μl of V. cholerae genomic DNA.
Url:
DOI: 10.1007/s11274-011-0978-x
Affiliations:
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unit="issue">4</biblScope><biblScope unit="page" from="1699">1699</biblScope><biblScope unit="page" to="1706">1706</biblScope></imprint><idno type="ISSN">0959-3993</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0959-3993</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Asymmetric PCR amplicon</term><term>Carbon screen-printed electrode</term><term>Chronoamperometry</term><term>Enzyme-based electrochemical genosensor</term><term>Probe-based sandwich-type hybridization</term><term>Vibrio cholerae</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A disposable horseradish peroxidase (HRP)-based electrochemical genosensor was developed for chronoamperometric detection of single-stranded asymmetric lolB gene PCR amplicon (118 bp in length) of the food-borne pathogen, Vibrio cholerae. A two-step sandwich-type hybridization strategy using two specific probes was employed for specific detection of the target single-stranded DNA (ssDNA). The analytical performances of the detection platform have been evaluated using a synthetic ssDNA (ST3) which was identical to the target single-stranded amplicon and a total of 19 bacterial strains. Under optimal condition, ST3 was calibrated with a dynamic range of 0.4883–15.6250 nM. By coupling asymmetric PCR amplification, the probe-based electrochemical genosensor was highly specific to the target organism (100% specificity) and able to detect as little as 0.85 ng/μl of V. cholerae genomic DNA.</div></front></TEI><affiliations><list><country><li>Malaisie</li><li>Thaïlande</li></country></list><tree><country name="Malaisie"><noRegion><name sortKey="Low, Kim Fatt" sort="Low, Kim Fatt" uniqKey="Low K" first="Kim-Fatt" last="Low">Kim-Fatt Low</name></noRegion><name sortKey="Chan, Yean Yean" sort="Chan, Yean Yean" uniqKey="Chan Y" first="Yean-Yean" last="Chan">Yean-Yean Chan</name></country><country name="Thaïlande"><noRegion><name sortKey="Chuenrangsikul, Kritsanaporn" sort="Chuenrangsikul, Kritsanaporn" uniqKey="Chuenrangsikul K" first="Kritsanaporn" last="Chuenrangsikul">Kritsanaporn Chuenrangsikul</name></noRegion><name sortKey="Rijiravanich, Patsamon" sort="Rijiravanich, Patsamon" uniqKey="Rijiravanich P" first="Patsamon" last="Rijiravanich">Patsamon Rijiravanich</name><name sortKey="Surareungchai, Werasak" sort="Surareungchai, Werasak" uniqKey="Surareungchai W" first="Werasak" last="Surareungchai">Werasak Surareungchai</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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